Analytical testing
HPLC vs Mass Spectrometry for Peptide Analysis
An educational comparison of two analytical methods commonly encountered in peptide documentation.
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Analytical testing
What HPLC tells you
Chromatographic methods can separate components and provide a basis for assessing the relative profile of a sample under the stated method.
Analytical testing
What mass spectrometry tells you
Mass spectrometry can provide molecular-mass information that helps with identity-related characterization under the method used.
Analytical testing
Why they are complementary
The methods answer different analytical questions. Buyers should read the method, result and stated specification together rather than treating one number as a complete quality assessment.
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